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Table 1.

Allele Frequency Measurements Across a Range of Values

Allele 1 frequency (by OD₂₆₀)		Expected ΔC_t		PON		B71
Allele 1 frequency (by OD₂₆₀)		Expected ΔC_t		average observed ΔC_t	heterozygote corrected ΔC_t	measured allele 1 frequency	standard deviation (±)	average observed ΔC_t	heterozygote corrected ΔC_t	measured allele 1 frequency	standard deviation (±)
0.95	−4.25	−4.01	−4.05	0.94	0.016	−3.21	−3.77	0.93	0.012
0.90	−3.17	−2.99	−3.03	0.89	0.010	−2.23	−2.79	0.87	0.023
0.80	−2.00	−1.88	−1.92	0.79	0.014	−1.22	−1.78	0.77	0.040
0.67	−1.00	−1.02	−1.06	0.68	0.051	−0.21	−0.77	0.63	0.032
0.59	−0.50	−0.44	−0.48	0.58	0.030	0.24	−0.32	0.56	0.031
0.50	0.00	0.14	0.10	0.48	0.016	0.70	0.14	0.48	0.024
Het.	0.00	0.04	0.00	0.50	0.051	0.56	0.00	0.50	0.013
0.42	0.50	0.78	0.74	0.37	0.045	1.21	0.66	0.39	0.016
0.33	1.00	1.18	1.14	0.31	0.007	1.65	1.09	0.32	0.021
0.20	2.00	2.11	2.07	0.19	0.007	2.54	1.99	0.20	0.011
0.10	3.17	3.21	3.17	0.10	0.014	3.63	3.07	0.11	0.007
0.05	4.25	4.52	4.48	0.04	0.003	4.68	4.12	0.05	0.002

[i] C_t measurements are average of four replicates.