Markup | Genome Research

Table 1.

Complete List of STSs Used for Contig Construction

STS name		Primer		Annealing temp. (°C)		Product size (bp)	Source
STS name		forward 5′ → 3′	reverse 5′ → 3′	Annealing temp. (°C)		Product size (bp)	Source
N877-13	GGACCTAACCAGATCCAGTA	GCAGACCAAATATCATCTCTC	55	135	Bova et al. (1996)
D8S1731[i]	CCAAGCAAATCATGGAAATC	AGCAAACTTATCCCACAAGG	55	220^b	Stanford G3 RH Map
C18-2644	CCTCTAAGTCCCTGTGTCAG	AAGCGCTAATGGGTCTCT	55	186	Bova et al. (1996)
324T7	GCTAAGATCACAGAGGGAACTGA	ATTGTGTTACGATTGCCGTG	55	146	end sequence of BAC 324C3[iii]
126SP6	GCCCTGCTTCAGCCTTATG	GCAGTCCAGTTTATTAGGTCCTG	55	416	end sequence of BAC 126O19[iii]
150E3-T7	TTATAGATTGAGGAGCTATGGTTGG	TGTATACATAGGTGTGTATACATAGGT	55	148	end sequence of BAC 150E3[iii]
N877-15	CACGTCTGGCCTTTATTATG	GTGAATGAACTGCTTTGAGG	55	288	Bova et al. (1996)
N33e1	GGCCCGCCCCCGACCACAG	CCCGGGGCGCTCCTTCACG	65	176	N33, exon 1 sequence[iv]
E6	TCCGAGGGCTATATACTTCC	CATCTTGTCTTTGCTCCAAT	55	101	Bookstein et al. (1994)
N33e3	ATGTGGCTGTGTCGCAATAAAAC	GGGCCATAGTAGAAACACATCAGT	55	217	N33, exon 3 sequence[iv]
D8S1992[ii]	TTCATCGTCTGAACCTGG	CAGGGTTGAAGCAATCTG	55	300^b	MacGrogan et al. (1996)
P10	TTGCAGAGGTTCAGGACAG	CATTCAGACAGATGTCAGAGTC	55	800^b	Bova et al. (1996)
N33e8	GCCAGGCTCAGTTTGTG	TGTAGCTTTAGTTTGCCCTTTTC	55	94	N33, exon 8 sequence[iv]
115A12-SP6	TGCCCCGTAACTTGAAATCT	AGCCATCATCACCTTGCTCT	55	160	end sequence of P1 115A12[iii]
J28	AGCTTCATCTTCAGAGCAAG	GTACGATAAGGGTGACCATC	55	222	gift from R. Bookstein (unpubl.)
N33e11	AGGGAACTGGATGAATGGT	TTGCTCGAAAATGGATAGTG	50	229	N33, exon 11 sequence[iv]
D8S549[i]	AAATGAATCTCTGATTAGCCAAC	TGAGAGCCAACCTATTTCTACC	55	170^b	Gyapay et al. (1994)
115A12-T7	CAGAAACCTTTGTTAACTGTCAG	CTTACCTCATATGCTCACACTGT	50	146	end sequence of P1 115A12[iii]
350A20-T7	AGTTTGGGGAGCTGTTTGTTTGAA	CGCGCCCGGCCTACTCTC	55	194	end sequence of BAC 350A20[iii]
60C12-T7	GAGCTGCTCCGAGAACTTACAT	GTCAGGCCTTCTACCAACAACAT	55	170^b	end sequence of P1 60C12[iii]
N19	GTGAAGGAGGGCAGTCAT	CAGGGTTGAAGCAATCTG	55	175	MacGrogan et al. (1996)
60C12-SP6	ACTCTGGTATACACTGATGTAATACAG	TTAGCTGAGACATTTTCTGAAG	55	125	end sequence of P1 60C12[iii]
Y8	GGGAAGCTATGGTAAAACAT	GGCAAGTAGATGTTACAGAAG	55	150^b	gift from R. Bookstein (unpubl.)
E17	CAAGGCATATCACAACTGC	GATAATTGAACTGTCACCTCTG	55	121	Bookstein et al. (1994)
YE766	GACTCTTGCCACCTTGTAAA	ATCTCCAAACCTACTTCTCC	55	89	Bookstein et al. (1994)
E1	TGACACACTTGCCATTTGAT	TTCCATTAGTCCCAGTTGTC	55	131	Bookstein et al. (1994)
D8S602[i]	GACTCTATTTTACTATCATTGACAG	GTTATCTTCTGCCTGGTGAAAAG	55	300^b	Takanishi et al. (1997)
45G06-SP6	GAGGAAAATTCTAAACAAAATGTT	AATTTGCCAGATCTTTAG	50	143	end sequence of P1 45G06[iii]
E2d	TGAAGCCATCTGTGGG	GTAAGAACTGTTAAGTGGAAAG	55	116	Bookstein et al. (1994)
MSR10	GTGGGAGCGGCCCTCACGAG	TGCACGGCTTGAACACCTGGGTAT	60	139	MSR, exon 10 sequence[iv]
84E02-T7	CTACCTTGCCACCTTCCAAAACTA	GAGAAGCCAACACTGAGGGAACTA	50	154	end sequence of COS 84E02[iii]
MSR5	TTAGAGGAGCGTGTTTACAATGT	GAATGTTCCCAATCTTTCAGTCT	55	143	MSR, exon 5 sequence[iv]
E3	GCCTGTTTCATCGAACC	CCTGGCATTCTTTACCTAGA	55	85	Bookstein et al. (1994)
GATA29A08	CCTGAGTCCAGGGAGGTC	CGGAAAAAACCCAAGTACCA	55	332	Stanford G3 RH Map
MSR[i]	TTCATCTATTGCATTCC	CAAAATTTCAGCATGACAACTG	50	102	Matsumoto et al. (1990)
D8S2066	TTTTCTCCATCCGGTGACTC	CCAACTACGGCATGGTTTCT	55	170^b	Stanford G3 RH Map
84E02-T3	CCCTGGTGAGACAGCGAGAC	AACTCACTCTTCACCCCATTTAGG	50	149	end sequence of Cos 84E02[iii]
E20	TGAATTTGCATAGTCTGCAG	CAGCTCTAACAAGGCTCCTA	55	107	Bookstein et al. (1994)
E56	TTTGTTGAGGACAAATACCC	TGTCACGATGAGGATTGTTA	55	170	Bookstein et al. (1994)

[i] Denotes polymorphic markers.

[ii] Approximate PCR product size.

[iii] Primers derived from end sequences of bacterial clones obtained in this study.

[iv] Primers designed based on published sequences.