Common DNA-binding factors promote termination of Pol II and lagging strand DNA synthesis. (A) Pile-up analysis of xPAT signal with Okazaki fragment 3′-ends for all tRNA genes functioning as Pol II roadblocks, centered on the upstream edge of TFIIIB binding sites. The black boxes labeled “A” and “B” correspond to the approximate locations of the box A and box B sequences bound by the transcription factor TFIIIC. Signal above the zero line represents the sense strand with respect to each tRNA gene, while signal below the zero line is anti-sense. (B) Comparison between roadblocked Pol II 3′-ends (xPATs) and roadblocked Pol δ 3′-ends (Okazaki fragments) for TFIIIB (left) and Reb1p (right) sites. The black bar indicates the TFIIIB footprint (left) and binding motif for Reb1p (right). (n) Number of roadblocks observed genome-wide for each class. For Reb1p, signal is oriented relative to the strand of roadblocked transcription.