Spatial interactions in a TNF-responsive locus. HUVECs were stimulated with TNF for 0, 30, or 60 min, and 3C-seq performed using the TSSs of the BMP4, CDKN3, and SAMD4A genes as viewpoints. (Top) Browser view, including ChIP-seq data, of 2 Mbp on Chromosome 14 (ideogram on top). Rectangles 1–4: TADs. Colored triangles: 3C-seq viewpoints. Arrows (bottom): RELA-bound enhancers contacted by BMP4 (white), CDKN3 (blue), and SAMD4A (maroon). (Bottom) Zoom-in on the interactions formed by TNF-suppressed BMP4 and TNF-induced SAMD4A TSSs. 3C-seq profiles are shown alongside JDP2 and RELA ChIP-seq data at 0 and 30 min post-stimulation.