Effects of the MKT1-30G and SAL1-nfs alleles. (A) Engineering of the MKT1-END3-SAL1 locus by double-cut CRISPR-Swap. In a BY strain, the MKT1 and SAL1 ORFs were replaced with KanMX and HphMX, respectively. These two cassettes were cut by CRISPR (red arrows), and the locus was replaced by the depicted alleles (variant positions are starred) provided on a PCR-amplified fragment. (B) Growth rates in liquid YPD. P-values are shown for comparing each genotype to the engineered BY control (G) and for the test of the epistatic interaction between the MKT1-30G and SAL1-nfs alleles (G×G). Box plots are orange if P < 0.05. Growth measurements from the same strain are connected by a vertical line. Box plots as in Figure 3. The median of the BY wild-type strain is depicted as a horizontal line through the plot. (C) Colony size of engineered strains after 40 h of growth on solid YPD. Box plots and P-values for G and G×G are as in B. (D) Colony sizes on solid YPD with lithium acetate. P-values for G×E interactions comparing the given condition to solid YPD are indicated for each genotype, along with that for the G×G×E term.