CRI-SPA-Map isolates contain tracks of W303 variants on ChrXIV-L. (A) Position of W303 tracts in four local (S for HygS) and two distal (R for HygR) isolates from four YKO strains. W303 tracts in all isolates are depicted in Supplemental File S1. BY alleles are in blue and W303 alleles in green. The sequence between the last W303 variant in the tract and the first BY variant could originate from either the BY or W303 strain and is therefore shown in gray. Targeted ORF positions are shown with dotted lines. Variant positions are marked by tick marks with the position of the telomere (TEL) and centromere (CEN) at the bottom of the plot. (B) Allele designation at each variant (not drawn to scale) in isolates of YNL077W. Variants within the ORF are in red. (C) The W303 variant tract at YNL077W ORF in isolate S1 (drawn to scale), with distances to the most distal W303 and nearest BY variants at each end of the ORF. (D) Cumulative distribution of 286 isolates from 69 YKO strains with the last W303 and first BY variant at the indicated distance from the 5′-end and 3′-end of the ORF. Vertical dashed lines designate the maximum (blue) and minimum (green) of the median tract lengths. For distal isolates, only the centromeric side was analyzed. Isolates with mosaic tracts or W303 tracts that do not extend beyond the ORF were excluded.
