Downsampling analysis for 80K-neurons data set. RNA is downsampled in equal proportions to the overall number of counts. Complexity (Lincoln index), the number of barcodes, as well as retained oligos and Pearson's correlation of oligo activity across replicates (median values across replicates or replicate comparisons). (A) RNA count downsampling in MPRAsnakeflow. Sampling is done before the assignment on the raw counts. (B) Downsampling using the MPRAlib on the overall output (after the assignment).