Immunoglobulin and major histocompatibility complex genes are anticorrelated in the immunoreactive (IMR) subtype, but not in the differentiated (DIF) or mesenchymal (MES) subtypes. Data visualization of predicted spatial domains using Seurat in select Denisenko Visium samples. We do not include the spatial domain visualization for sample SP4 (DIF) here, as it displays much less variability in spatial domains in comparison to other DIF samples. For SP4 spatial domains, please see Supplemental Figure S11. (A) Sample ID and color-coded molecular subtype: DIF in orange, IMR in green, and MES in purple). (B) Spearman's correlation values between domain 4 DEGs, major histocompatibility complex (MHC) genes and immunoglobulin (IG) genes. There was a negative correlation between MHC and IG exclusively for the IMR samples. (C) Column corresponding to the Seurat module score of DEGs identified from domain 4. (D) Column corresponding to the Seurat module score (average gene program expression) of major histocompatibility complex coding genes. (E) Column corresponding to the Seurat module score of immunoglobulin coding genes. Note that spots tend to coexpress high MHC and IG in the DIF and MES samples (top and bottom two rows), whereas spots expressing high MHC tend to anticolocalize with spots expressing high IG in IMR samples (rows color-coded green). (F) Integrated clustering scheme in space for reference.