Geographical origin and experimental workflow for the different batches of the Nomascus siki cell line analyzed in the present study. As shown in panel A, all three batches originated from the University of Cambridge: batch1 (purple) was directly transferred to Uniba (Italy); batch2 (green) and batch3 (red) were first shipped to UTAD (Portugal) and subsequently to Uniba for further processing and analysis. Panel B illustrates the processing of all the batches at Uniba (Italy), specifying culturing timelines, with the number of passages indicated in parentheses, the production of frozen aliquots, and metaphase preparations for each batch, along with the sequencing techniques applied to them.