AccuSNV achieves high precision across simulated data sets with different sequencing depths. Precision, recall, and average F1 scores of AccuSNV and seven other variant-calling tools were evaluated across simulated bacterial data sets at five sequencing depths (10×, 20×, 30×, 40×, and 50×). Axes use a transformed scale (−log10(1.0001 − x)) to enhance visualization in the higher range (0.9–1). Shapes represent species and colors represent tools. The vertical line at 0.99 precision highlights how more tools achieve high performance at greater sequencing depths. Top panels show precision–recall scatter plots for all tools and species at each depth. Middle panels provide a zoomed-in view of precision distributions across tools and species. Bottom panels summarize the average F1 score for each tool. The bar corresponding to AccuSNV is highlighted by a black outline.
