Overview of the spa-ChIP-seq workflow and evaluated ChIP-seq experimental conditions. The automated steps of the ChIP-seq workflow are highlighted in the gray boxes of the flowchart on the left and the evaluated experimental conditions in the yellow boxes. The green box shows the required manual preparations of master mixes prior to the start of each automated step. The cross-linking reagent formaldehyde (FA) is represented as a small blue dot, and disuccinimidyl glutarate (DSG) is represented as a large red dot.