Comparison of mutational trinucleotide context and COSMIC single-base signatures across ART-derived and natural-born G2 samples. (A) Bar plot of the relative contribution of COSMIC single-base signatures to the proportion of observed dnSNVs in the ART and natural birth cohorts. Permutation testing indicates no significant differences in signature contributions between cohorts. (B) Heatmap displaying the ratio of the average proportions of each of the 96 trinucleotide mutational contexts between ART-derived and natural-born samples. White boxes indicate that the mutational type was absent in both cohorts, and gray hatching indicates that the mutation type was only present in ART samples and was absent in natural-born G2 mice, rendering ratios as undefined. The asterisk indicates a significant difference in the C[C>A]A trinucleotide mutation fraction between the two cohorts.
