Alternative splicing mechanisms associated with RNA isoform switches and consequences for protein accumulation. (A) Number of events associated with particular functional implications to the RNA isoform. (B) Statistical significance of events related to selected functional consequences to the RNA isoform. (C) Illustration of alternative transcription and splicing events. (D) Number of events with identified alternative transcription or splicing events during the iPSC-to-iCM differentiation. (A3) Alternative 3′ splice site, (A5) alternative 5′ splice site, (ATSS) alternative transcription start site, (ATTS) alternative transcription termination site, (ES) exon skipping, (IR) intron retention, (MES) multiple exon skipping, and (D0, D2, D4, D30) days of differentiation.