Histological analysis of cardiac development in Gata6 and Por mutant mice
| WT | Gata6−/+ | Por−/+ | Gata6−/+ Por−/+ | |
|---|---|---|---|---|
| E18.5 | ||||
| Samples no. | 2 | 4 | 2 | 5 |
| ASD only | 0 | 0 | 0 | 1 (20%) |
| ASD+VSD | 0 | 0 | 0 | 1 (20%) |
| Normal | 2 (100%) | 4 (100%) | 2 (100%) | 3 (60%) |
| P0 | ||||
| Samples no. | 4 | 7 | 7 | 12 |
| ASD only | 0 | 0 | 0 | 7 (58%) |
| ASD+VSD | 0 | 0 | 0 | 0 |
| Normal | 4 (100%) | 7 (100%) | 7 (100%) | 5 (42%) |
| Total (% normal) | 6 (100%) | 11 (100%) | 9 (100%) | 17 (47%) |
[i] Although 100% of wild-type, Gata6−/+, and Por−/+ mice developed normal hearts, compound heterozygous Gata6−/+ Por−/+ mice exhibited CHDs, including atrial-septal defects (ASDs) alone or in addition to ventricular-septal defects (VSDs). At embryonic day 18.5 (E18.5), two out of five embryos displayed ASD or both ASD and VSD. At postnatal day 0 (P0), seven out of 12 pups presented with ASD.