Expression through ontogeny of MHC-I genes annotated in the genomes (data set G). Relative expression, that is, the fraction of all RNA-seq reads in a library mapping to all MHC-I genes and pseudogenes in the assembly that mapped to a particular gene sequence, is shown. Box plots show medians, interquartile, and total ranges. Gray stripes indicate genes that show higher relative expression in larvae than in adults—those may be nonclassical (MHC-Ib) genes. Species abbreviations: (Lh) L. helveticus, (Lv) L. vulgaris, (Pw) P. waltl, (Tc) T. cristatus.