Chromosome-level genome synteny and characterization of the macro- and microchromosomes in gar species. (A) Comparison of whole-genome alignment synteny segments among A. spatula, L. osseus, and L. oculatus. (B) Comparison of interaction decay exponents between macrochromosomes and microchromosomes in A. spatula and L. osseus. (C) Comparison of substitution rates between macrochromosomes and microchromosomes in gars. (D) Comparison of macrosynteny conservation across diverse phylogenetic lineages. The conservation index (CI), Oxford dot plots, and gene synteny plots all indicate that A. spatula microchromosomes exhibit higher conservation than macrochromosomes throughout evolution. Colored highlights indicate microchromosomes.