Statistical analysis of exon orientation and position in raw reads
| Region | Coverage | F/R orientation | Exon position (F) | Exon position (R) | |||
|---|---|---|---|---|---|---|---|
| Count | P | AD stats | P | AD stats | P | ||
| CCY exon 2 | Low | 3/10 | 0.092 | 3.75 | 0.013 | 1.73 | 0.131 |
| kl-5 exon 14 | Low | 6/6 | 1.000 | 8.76 | <10−3 | 4.67 | 0.005 |
| kl-5 exons 3–10 | Low | 3/9 | 0.146 | 2.36 | 0.063 | 7.63 | <10−3 |
| kl-3 exons 15–16 | Low | 6/10 | 0.455 | 10.05 | <10−3 | 20.04 | <10−3 |
| ORY exons 1–2 | Low | 4/12 | 0.077 | 0.62 | 0.621 | 1.4 | 0.204 |
| Ppr-Y exon 3 | Low | 3/15 | 0.008 | 8.62 | <10−3 | 4.26 | 0.007 |
| Pp1-Y1 | Normal | 87/76 | 0.434 | 0.86 | 0.436 | 0.72 | 0.539 |
| Pp1-Y2 | Normal | 53/47 | 0.617 | 0.33 | 0.915 | 0.48 | 0.767 |
| Ppr-Y exon 4 | Normal | 111/118 | 0.692 | 1.84 | 0.113 | 2.19 | 0.072 |
| kl-5 exons 11–13 | Normal | 69/70 | 1.000 | 33.66 | <10−3 | 33.14 | <10−3 |
[i] Note that low-coverage regions frequently display skewed exon orientation (F/R; columns 3, 4) and nonrandom exon positions (columns 5–8).