SETD3 and ACTB interact with several large transcription complexes. (A) Chromatin and nuclei were purified from HCT-116 Ct cells followed by immunoprecipitation with either SETD3 or ACTB specific antibodies. Coimmunoprecipitated proteins were then identified by mass spectrometry. (B) Bubble plot showing enriched biological processes associated with the 161 shared proteins. The x-axis indicates the number of proteins per category, and the y-axis lists significantly enriched processes. Bubble size reflects the number of proteins assigned to each category, and bubble color corresponds to statistical significance (–log₁₀ P-value), ranging from blue (less significant) to red (highly significant). Underlined enriched categories include RNA processing, gene expression, nucleosome/chromatin organization, and chromatin remodeling. (C) STRING analysis of the 161 shared proteins found in A. Nodes represent individual proteins and edges represent known or predicted interactions. Proteins are grouped into functional modules highlighted by colored ellipses. The visualization illustrates that shared proteins are enriched in interconnected modules involved in RNA metabolism, chromatin regulation, mitochondrial function, and protein synthesis.