Evaluation of the presented targeted demethylation approach on high-confident m⁶A targets with <0.5 methylation stoichiometries. SELECT-qPCR data and normalized m⁶A abundance are shown for CDC37 (NM_007065.4 m⁶A:1145; A), PSAT1 (NM_058179.4 m⁶A:1375; B), and MRPL14 (NM_032111.4 m⁶A:476; C) at 48 h posttransfection with Dem6A-Vec expressing the target gRNAs.