Demethylation impact of Dem6A-Vec at 48 h posttransfection. (A) Amplification plots from the implementation of SELECT-qPCR on the m⁶A:2886 of EEF2 and a nonmethylated site of the housekeeping HPRT1 gene. (B) Amplification plots from the implementation of SELECT-qPCR on the m⁶A:418 of RRAGA and a nonmethylated A site of the housekeeping HPRT1 gene. (C) Melt curves generated from the SELECT-qPCR assays on the EEF2 m⁶A:2886 and RRAGA m⁶A:418 sites.