Schematic illustration of Dem6A-Vec. (A) Circular map of Dem6A-Vec. The distinct features of the plasmid are shown in different colors. The location, size, and type of each feature are also exhibited. (B) Linear map of Dem6A-Vec. The plasmid sequence encompasses two recognition sites for the type IIS PaqCI restriction endonuclease, thus enabling the scarless cloning of any RfxCas13d-compatible gRNA of choice through golden gate cloning. (C) Fluorescence microscopy images highlighting the cellular localization of Dem6A-Vec.