Total number of genes annotated in the six cactophilic Drosophila species sequenced in this work
| Species/subspecies | Total genes | Host localization, acceptance, and usage genes | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Localization | Acceptance | Host usage | ||||||||
| OBP | OR | GR | IR | ABC | EST | GST | UDP | CYP | ||
| Ref. D. m. wrigleyi | 15,045 | 25 | 59 | 45 | 22 | 27 | 17 | 28 | 21 | 59 |
| D. m. mojavensis | 14,810 | 25 | 59 | 44 | 22 | 27 | 17 | 27 | 20 | 59 |
| D. m. wrigleyi | 14,948 | 25 | 59 | 45 | 22 | 27 | 17 | 27 | 20 | 59 |
| D. m. sonorensis | 14,808 | 25 | 57 | 44 | 22 | 27 | 17 | 27 | 20 | 58 |
| D. arizonae | 14,441 | 25 | 56 | 41 | 21 | 27 | 17 | 27 | 20 | 59 |
| D. buzzatii | 18,050 | 25 | 52 | 34 | 21 | 27 | 16 | 27 | 20 | 68 |
| D. koepferae | 17,848 | 25 | 45 | 34 | 24 | 27 | 13 | 25 | 23 | 62 |
[i] The “Ref. D. m. wrigleyi” represents the annotation in the reference genome used to perform the gene annotation for our long-read ONT genomes. HLAU genes are separated according to their functions: localization—odorant-binding proteins (OBP) and odorant receptor (OR), acceptance—gustatory receptor (GR) and ionotropic receptor (IR), and host usage—ATP-binding cassette (ABC), carboxylesterase (EST), glutathione S-Transferase (GST), glycosyltransferase (UDP), and cytochrome P450 (CYP).