Table 1.

Summary of genome annotation methods evaluated in this study

MethodMethod typePredictions
BRAKER1Ab initioProtein-coding transcripts
BRAKER2Ab initioProtein-coding transcripts
BRAKER3Ab initioProtein-coding transcripts
CGPproteinAb initioProtein-coding transcripts
CGPRNA-seqAb initioProtein-coding transcripts
MAKERAb initioProtein-coding transcripts
StringTie + HISATRNA-seq assemblerTranscriptsa
StringTie + STARRNA-seq assemblerTranscriptsa
Scallop + HISAT2RNA-seq assemblerTranscriptsa
Scallop + STARRNA-seq assemblerTranscriptsa
TOGALiftoverProtein-coding transcripts
LiftoffLiftoverProtein-coding and noncoding

[i] aWhen paired with TransDecoder, produces CDS and UTR annotations.