Ancestral gene duplications of ECM-induced gene families. (A) CAFE results for three ECM-induced gene families that are significantly expanded on the stem branch of Tuberaceae. For each node, inferred and observed gene-family counts are shown on internal and terminal branches, respectively. Green indicates expansions, red indicates contractions, and significant changes are marked with an asterisk. Numbers in brackets indicate node IDs and refer to Supplemental Table S8. Species abbreviations are given in Figure 3. (B) InterProScan domain annotations of representative T. panzhihuanense proteins from each of the three gene families. For OG0000075, we used a reference protein from T. magnatum because no family members were detected in T. panzhihuanense. Symbols correspond to those in A. (TPR) Tetratricopeptide repeat. Supplemental Figure S17 shows the results for the two others significantly expanded ECM-induced gene families, OG0000009 and OG0000138, encoding ankyrin repeat–containing proteins and AAA ATPases, respectively.