Late Cretaceous emergence of Tuberaceae and a Paleogene diversification of true truffles. Phylogenetic relationships and divergence time estimations obtained with 1057 complete and single-copy BUSCO genes. The species tree was constructed using an amino acid supermatrix, and both protein (black) and nucleotide (red) data were used for divergence time estimation. All nodes received maximum support values from the species tree inference, except for the split between Arthrobotrys and Pezizomycetes. The outgroup Schizosaccharomyces osmophilus was removed for visualization purposes. Numbers within circles represent the calibration points described in Supplemental Table S10. (Ne) Neoproterozoic, (Cam) Cambrian, (Or) Ordovician, (Si) Silurian, (De) Devonian, (Car) Carboniferous, (Pe) Permian, (Tr) Triassic, (Ju) Jurassic, (Cr) Cretaceous, and (Pa) Paleogene.