Sanger validation of high-confidence nonsynonymous DESs with significantly lower editing levels in GF compared to other conditions. Sanger traces for representative samples were displayed. Boxplots show the comparison of Sanger editing levels between 12 CV and 12 GF. (A) Phosphatidylinositol-binding clathrin assembly protein (PICALM) LAP. (B) Split ends protein spen. (C) Ly-6 protein qvr. Note that 12 CV and 12 GF samples were subjected to PCR, but only nine CV and six GF samples were successfully sequenced. (D) Voltage-gated potassium channel protein Shab and leukocyte receptor member 8.