Validation of the gut bacteria–Adar–editome axis using brain transcriptomes of single bacterial inoculation. (A) Experimental design of the public data. The CV and GF bees were defined similarly to our study. The monocolonized bees were generated by inoculating a single microbial species (F4, F5, or Gi) to the GF bees, respectively. (B) Comparison of global RNA editing levels. Reads from three biological replicates were pooled. Wilcoxon rank-sum tests were used to determine the significance. (C) Comparison of the Adar p.482 Ile > Met editing level. Reads from three biological replicates were pooled. Fisher's exact tests were used to determine the significance. (D) IGV visualization of the Adar p.482 Ile > Met editing level in public transcriptomes. (E) Venn diagram showing the overlap of downregulated nonsynonymous editing sites in GF from different comparisons. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; (n.s.) not significant.