DMRs and differential expression. (A) The proportion of DM-DEGs with hyperentropic (ΔS > 0.1) and iso- or hypoentropic (ΔS < 0.1) DMRs. Results are reported for hyper- (Δβ > 0.2) and hypomethylated (Δβ < −0.2) DMRs overlapping CGIs and outside CpG islands (NoCGIs). Textured bars show the number of DM-DEGs with iso- or hypoentropic (ΔS < 0.1) DMRs. (B) The results of ORA for DM-DEGs. The analysis was performed separately for genes affected by DMRs at the 5′ regulatory region (5′Reg), the gene body (GB), and the 3′ UTR and for DMRs overlapping CGIs and NoCGIs. The blocks of the corrplot report the results of ORA for different classes of DMRs formation: Δβ > 0.2 and ΔS < 0.1 (hypermethylated and hypo- and isoentropic), Δβ > 0.2 and ΔS > 0.1 (hypermethylated and hypo- and isoentropic), Δβ < −0.2 and ΔS < 0.1 (hypomethylated and hypo- and isoentropic), and Δβ < −0.2 and ΔS > 0.1 (hypomethylated and hypo- and isoentropic). The color intensity in each cell reflects the statistical significance according to the colorbar. For each category, Fisher's exact tests were calculated comparing the number of DM-DEGs, DMGs, DEGs, and all genes tested in RNA-seq experiments. (C) The results of ORA for underexpressed DM-DEGs. The analysis was performed separately for genes affected by DMRs at the 5′ regulatory region (5′Reg), the gene body (GB), and the 3′ UTR and for DMRs overlapping CGIs and NoCGIs. The blocks of the corrplot report the results of ORA for different classes of DMRs formation: Δβ > 0.2 and ΔS < 0.1 (hypermethylated and hypo- and isoentropic), Δβ > 0.2 and ΔS > 0.1 (hypermethylated and hypo- and isoentropic), Δβ < −0.2 and ΔS < 0.1 (hypomethylated and hypo- and isoentropic), and Δβ < −0.2 and ΔS > 0.1 (hypomethylated and hypo- and isoentropic). The numbers in each cell represent the total number of DM-DEGs for each category, and the color intensity reflects the statistical significance according to the colorbar. Fisher's exact test and number of genes were calculated for cancer-related pathways selected from KEGG database, TFs, TSGs, and oncogenes selected by COSMIC and GEAR genes.