UL N50s were obtained using the new ULK114 kit from three cell lines. (A) Read length distribution of GM12878 library run with the earlier ULK114 script version (left panel), while the other two libraries (middle and right panels) were run with the most current 5 kHz version of the script. Vertical dashed black lines indicate N50s. (B) Run metrics of the three libraries showed that the Nemo protocol performed better than ONT's.