Combinatorial effects of extraction and library cleanup methods, either with or without the use of kits, on the UL sequencing outputs. (A–I) Read length distributions of the libraries: Graph titles denote the used extraction kit/method and the cleanup protocol; dashed black vertical lines denote N50s. (J) Sequencing metrics after 6 h of run (excluding the first 10 min). DNA was extracted from GM12878 cells. Each library was loaded on a MinION R9.4.1 flow cell and run on the GridION platform.