Choosing the best precipitating agent that ensures fast homogenization. (A) Read length distribution of libraries precipitated with different buffers; dashed black vertical lines denote N50s. (B) Sequencing metrics after 6 h of run (excluding the first 10 min). All libraries were extracted from GM12878 cells using Monarch direct lysis protocol with an input DNA concentration of ∼40 ng/µL split into three cleanup preparations, loaded on MinION R.9.4.1 flow cells and sequenced on GridION platform.