Validation of the FindingNemo protocol. Sequencing outputs between rapid kit-based library preparation protocols (ULK001-Nemo and RAD004-Nemo protocol) show comparable performance when compared to the control ULK001 protocol. (A) Workflow of the library preparation protocols, (B) total yields subcategorized by read lengths, (C) time lapse of yields, (D) read length distributions (dashed black vertical lines denote N50s), and (E) time lapse of relative pore occupancies. Each library was loaded three times (i.e., total DNA equivalent to 3 million GM12878 cells) on a MinION R.9.4.1 flow cell following a nuclease flush protocol. All data are after 65 h of sequencing on the GridION platform.