Summary of the FindingNemo toolkit. (A) Similarities and differences between the standard and UL protocols that center on the way UHMW DNA is handled. Created with BioRender (https://www.biorender.com). (B) The route of FindingNemo toolkit consists of five main steps, with options at the extraction, library preparation, and cleanup steps. Options with asterisks (*) are our current laboratory workflow for the UL protocol.