The dismantling of telomere hyperclusters during return to growth follows but is independent of transcriptional activation. (A) Telomere hypercluster dismantling upon quiescence exit. Representative images of Rap1-GFP sampled during quiescence release. Brightness for all Rap1-GFP images is set at 1600 maximum. (B) Telomere hypercluster dismantling in rich medium (YPD) after shifting cells grown at 25°C to restrictive temperature (37°C; Methods) to inactivate RNAPII (rpb1-1) or not (WT): representative Rap1-GFP images after 15 or 30 min of refeeding of stationary phase cells. Brightness for all Rap1-GFP images is set at 1600 maximum.