Table 1.

Genes that affect mouth-form development in Pristionchus pacificus

Ppa GeneEl Paco v2 annotationChrProtein functionMutant lineMouth form (NGM)Mouth form (S-medium)References
Wild typePS312/RS2333>90% Eu>90% St
eud-1PPA43535XSulfatasetu445, tu442, tu450, RS31110% Eu—Ragsdale et al. 2013
seud-1/sult-1PPA12547ISulfotransferaseiub7, iub8, tu1061, tu1232100% Eu0% StBui et al. 2018; Namdeo et al. 2018
nag-1PPA06134XN-acetylglucosaminidasetu1137, tu1142100% Eu0% StSieriebriennikov et al. 2018
nag-2PPA34489XN-acetylglucosaminidasetu1138, tu1143—>90% StSieriebriennikov et al. 2018
sul-2.2.1PPA06135XSulfatase—>90% Eu100% StRagsdale et al. 2013; Sieriebriennikov et al. 2018
daf-12PPA28648aINuclear hormone receptortu381, tu389, tu39085% Eub—Bento et al. 2010
lsy-12ppa_stranded_DN30317_c2_g1_i3IVHistone acetyltransferasetu3192% Eu—Serobyan et al. 2016
mbd-2PPA09682VMethyl-binding proteintu3650% Eu—Serobyan et al. 2016
nhr-40PPA22002aXNuclear hormone receptortu505, tu1421, tu1418, tu14190% Eu100% StKieninger et al. 2016; Sieriebriennikov et al. 2020
nhr-1ppa_stranded_DN28508_c0_g1_i2XNuclear hormone receptortu515, tu1163, tu11640% Eu—Sieriebriennikov et al. 2020
daf-19ppa_stranded_DN25985_c0_g1_i1IITranscription factorRS3011 (tu1035), RS3238 (tu1167)10% Eu100% StMoreno et al. 2018, 2019
daf-21ppa_stranded_DN30790_c0_g1_i2IHeat-shock proteintu51952% Eu—Sieriebriennikov et al. 2017
osm-1PPA24556aIIntraflagellar transport proteinRS3170 (tu1129)5% Eu100% StMoreno et al. 2019
osm-12PPA23445IIIBBSome proteintu1099, tu1414100% Eu10% StMoreno et al. 2019
xbx-1PPA21711IDynein proteinRS3117 (tu1081)100% Eu70% StMoreno et al. 2019
ifta-1PPA23338IIIntraflagellar transport proteinRS2996 (tu1045)55% Eu97% StMoreno et al. 2019
dyf-1PPA06701VIntraflagellar transport proteinRS3092 (tu1072)35% Eu98% StMoreno et al. 2019
che-11ppa_stranded_DN27945_c0_g1_i1IIIntraflagellar transport proteinRS3121 (tu1085)75% Eu98% StMoreno et al. 2019
che-3PPA11202VDynein proteinRS3141 (tu1100)33% Eu100% StMoreno et al. 2019
osm-3;klp-20PPA03370;a

ppa_stranded_DN31699_c4_g13_i1		IV; IIIKinesin proteinsRS318 (tu1090; tu1131)33% Eu100% StMoreno et al. 2019
daf-25ppa_stranded_DN18164_c0g1_i1IIIMYND domain proteintu716Lenuzzi et al. 2023
tax-2PPA07435VNucleotide-gated channel subunittu1291, tu1440>80% Eu—Lenuzzi et al. 2023
daf-16PPA39986VTranscription factortu1514, tu151543% Eu—Lenuzzi et al. 2023
daf-11PPA14907IGuanylyl cyclasetu722, tu1438, tu1439, tu144080% Eu—Lenuzzi et al. 2023
dpy-6PPA38488IMucin-type proteinRS3887 (tu743)>90% Eu—Sun et al. 2022a
chs-2PPA39501IIChitin synthaseRS3911(tu1651), RS3912 (tu1652)Eu (toothless)—Sun et al. 2022b
daf-7.1;daf-7.2PPA36793; PPA27957III; XTGF-β signaling ligandRS4058 (tu1566; tu1573)60% Eu—Lo et al. 2022
tag-68ppa_stranded_DN25910_c0_g2_i3aVI-Smad transcriptional regulatorRS3791 (tu1557), RS3792 (tu1558)52% Eu—Lo et al. 2022
nas-6PPA30034IVAstacin metalloproteaseRS3913 (tu1653)60% Eu—Ishita et al. 2023
spr-5ppa_stranded_DN16305_c0_g1_i1VH3K4me1/2 demethylasecbh49, cbh81, cbh82, cbh14950% Eu—Levis and Ragsdale 2023
mdt-15.1ppa_stranded_DN24725_c0_g1_i8IIITranscriptional coregulatoryiub9, iub10, iub12Eu (aberrant)—Casasa et al. 2023
pddl-1ppa_stranded_DN27845_c0_g3_i3cIVDelta-9 desaturaseRS4411 (tu2028), RS4412 (tu2029)>100% Eue—Piskobulu et al. 2025
pddl-3PPA40514IVDelta-9 desaturaseRS4401 (tu2033), RS4402 (tu2034)d>90% Eue—Piskobulu et al. 2025
pddl-4ppa_stranded_DN27845_c0_g2_i1cIVDelta-9 desaturaseRS4398 (tu2030), RS4399 (tu2031)d>90% Eue—Piskobulu et al. 2025
dhs-28.1PPA20393XPeroxisomal beta- oxidationRS4147 (tu1855)100% Eue—Piskobulu et al. 2025
daf-22.1;daf-22.2PPA39143;a PPA41516II; IVThiolase; thiolaseRS2770 (tu489; tu504)100% Eue—Markov et al. 2016; Piskobulu et al. 2025

[i] Gene IDs are based on the El Paco v2 gene annotation that was used for this transcriptomic analysis.

[ii] aThese genes have different gene IDs in the updated gene annotation (El Paco version 3).

[iii] bThis is our observed mouth-form frequency (Supplemental Fig. S18), which differs from the previously reported percent Eu.

[iv] cThese genes are not annotated in El Paco version 2, so this is the El Paco version 3 annotation.

[v] dSee reference for additional mutant alleles of these genes.

[vi] eMouth form of these mutants is less responsive to high glucose.