Genome-wide landscape of chromatin occupancy for 201 transcriptional regulator mutants. (A) Functional classification of the 201 mutants in our MNase-seq data set. Labels are derived from the Saccharomyces Genome Database. (B) Schematic illustrating chromatin occupancy profiling of 201 yeast mutants via MNase-seq. MNase digests unprotected DNA, leaving DNA fragments bound by DNA-binding proteins such as nucleosomes or TFs. The resulting fragments are purified, sequenced, and plotted as a function of length, allowing nucleosome and TF occupancy to be visualized. Fragment midpoints are plotted along the x-axis, and fragment length is plotted on the y-axis, indicating nucleosome or TF-sized DNA. (C) Genome-wide map of chromatin changes in response to genetic perturbations. Loci with significant chromatin changes in response to a deletion are highlighted in purple (those reflecting the mutant deletion itself are excluded). Each row represents a mutant, and the x-axis represents genomic locations (base pairs) grouped by chromosome. (D) Deletion of CBF1 results in a loss of TF occupancy and inward shifting of nucleosomes at the MET10 locus (in contrast, this effect is not seen when BAS1 or MBP1, e.g., are deleted). (E) Deletion of ROX1results in a disruption and loss of nucleosome occupancy at the ANB1 locus. (F) Deletion of ARG80 and ARG81 both result in a loss of TF occupancy at the promoter in the ARG8 locus, with the loss more pronounced in the ARG80 mutant.