MutSTARR-seq results comparing enhancer activity (log₂fc) between the reference and alternate alleles. Box plots represent the distribution of activity across four technical replicates. Enhancer activity is defined by log₂ fold change, which represents the normalized output/input ratio in log₂ space. Variants that had a significant effect on enhancer activity (P < 0.05) are boxed in green. P-values were calculated using a t-test comparing the log₂fc(output/input) of the reference and alternate alleles across the replicates. (log₂fc) log₂ fold change, (SNV) single nucleotide variant.