Markup | Genome Research

Table 1.

DNA sequencing overview

A. PacBio Sequel II (CLR)
	Total bases (Gb)	No. of sequences	Max length (bp)	N50 (bp)	N90 (bp)
All reads	170.06	8,503,777	521,561	29,768	11,540
Guanapo	85.91	4,162,756	397,496	29,755	11,719
Maculatus	84.01	4,094,477	521,561	29,814	11,452
Unclassified	0.01	7328	22,512	1688	1097

B. Illumina (2 × 150 bp, PCR-free)
Maculatus XY female	146 M cluster	44 Gb
Guanapo XY male	146 M cluster	43 Gb

C. Assembly statistics^a
	Total bases (Mb)	No. of sequences	Max length (bp)	N50	L50	BUSCO completeness	BUSCO duplicated
Guanapo	745.3	23	46,441,515	34,623,888	11	98.00%	2.60%
Maculatus	745.2	23	46,441,515	34,623,888	11	97.90%	2.60%

[i] ^aThese final genomes were assembled using the same reference genome as guide, resulted in highly similar statistics.