Assessing SeqSplice output reproducibility. (A) Control experiment using a BRCA2 exon 4 WT construct to assess reproducibility of transcript identification and abundance across a range of cDNA template input amounts and PCR cycles. (B) Control experiment using a BRCA1 c.188T>C variant construct to assess reproducibility of transcript identification and abundance across a range of cDNA template input amounts and PCR cycles.