Sample performance
| Yield (Gb) | N50 (bases) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Barcode | Seq. | Unb. | Seq. | Unb. | Sample | Panel | Gene number | Off target Med. Cov. | Target Med. Cov. | Fold enrichment |
| 01 | 0.334 | 3.47 | 8149 | 555 | GM12878 | TruSight 170 tumor panel | 170 | 1 | 12 | 12 |
| 02 | 1.24 | 4.84 | 7191 | 552 | NB4 | TruSight RNA fusion panel | 508 | 1 | 15 | 15 |
| 03 | 1.25 | 3.84 | 6858 | 556 | 22Rv1 | COSMIC | 717 | 1 | 12 | 12 |
| Unclassified | 0.170 | 3.66 | 923 | 792 | * | * | * | 0 | 1 | * |
| Total | 2.99 | 15.80 | 5780 | 614 | 1 | 12 | ||||
| 05 | 1.28 | 14.76 | 7163 | 917 | GM12878 | TruSight 170 tumor panel | 170 | 4 | 35 | 9 |
| 06 | 4.36 | 23.39 | 7349 | 919 | NB4 | TruSight RNA fusion panel | 508 | 8 | 52 | 7 |
| 07 | 3.01 | 13.63 | 6999 | 923 | 22Rv1 | COSMIC | 717 | 4 | 27 | 7 |
| Unclassified | 0.703 | 15.50 | 543 | 989 | * | * | * | 4 | 5 | * |
| Total | 9.35 | 67.29 | 5514 | 937 | 4 | 31 | ||||
[i] Run metric performance per barcode and over the entire flow cell. Metrics are derived from real-time monitoring with minoTour (Munro et al. 2022), and from analysis of the final data set. Barcodes 01–03 were run on a single GridION flow cell; barcodes 05–07 were run on a single PromethION flow cell. (Seq.) Sequenced; (Unb.) unblocked; (Med. Cov.) median coverage. (*) Not applicable.