R2C2 and Illumina RNA-seq detect largely the same genes at similar levels. Gene-level saturation curve analysis of R2C2 data (A) and Illumina RNA-seq data (B). (C) Comparison of genes detected by either R2C2 or Illumina RNA-seq or both. (D) Expression levels as determined by R2C2 and Illumina RNA-seq for genes detected by either R2C2 or Illumina RNA-seq or both (colors as in A). (E) Pearson's correlation between gene expression values was determined by R2C2 and RNA-seq for each tissue. (F) Scatterplot of kidney gene expression values as determined by R2C2 and Illumina RNA-seq. (SC) spinal cord, (St) stomach, (SM) skeletal muscle, (SG) salivary gland.