Summary of germline heterozygous variant coverage provided by each scRNA-seq technology
| Sample | Sequencing technology | Number of reads | Number of variants called | % Variants covered by 1% or more cells | % Variants covered by 10% or more cells | % Variants present in scRNA-seq |
|---|---|---|---|---|---|---|
| Patient 3, Pretreatment | Sequel II | 13,045,554 | 13,403 | 12.61 | 1.73 | 43.58 |
| Patient 1, Posttreatment | Revio | 41,177,852 | 13,709 | 18.59 | 3.85 | 50.12 |
| Short-read 1 | Short read | 713,605,274 | 12,954 | 7.61 | 1.59 | 33.08 |
[i] Read counts come from segmented reads after barcode correction and UMI deduplication for HiFi sequencing and only reads that were mapped to the genome. The percentage of germline heterozygous variants identified with WES detected in each scRNA-seq is also shown.