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Table 1.

Long-read scRNA-seq metrics for each sample across the six patients

Patient	Time point	Sequencer	Cells recovered	Total HiFi reads	Average HiFi read length	Average HiFi read quality	Segmented reads	Segmented reads after barcode correction and UMI deduplication	Average segmented read length	Average reads per cell	Median UMIs per cell	Median genes per cell	Median transcripts per cell
1	Pretreatment	Revio	4384	6,459,939	10,397	30	62,282,311	34,303,737	897	12,300	7001	1107	1317
1	Relapse	Revio	4527	6,305,954	13,674	30	92,245,332	41,177,852	885	17,610	8110	1130	1360
2	Pretreatment	Revio	5357	5,093,438	14,308	30	73,572,836	46,692,179	930	12,539	8038	1259	1540
2	Relapse	Revio	4466	3,046,815	13,338	31	38,840,774	30,954,010	934	8245	5709	897	1070
3	Pretreatment	Sequel II	3025	1,149,291	15,027	32	12,656,112	7,629,323	1158	3490	1866	294	522
3	Relapse	Sequel II	4702	1,518,018	16,691	31	21,029,102	9,086,104	1098	3566	1704	309	344
4	Pretreatment	Sequel II	5337	1,354,734	16,696	32	18,975,741	13,045,554	1104	3147	2235	535	590
4	Relapse	Sequel II	3940	1,149,151	15,178	32	15,146,244	9,103,200	1053	3250	2011	423	472
5	Pretreatment	Revio	7288	1,983,307	13,978	30	27,877,526	21,872,099	907	3473	2302	412	460
5	Relapse	Revio	9372	3,609,493	10,797	30	45,847,606	32,198,895	725	4140	2799	407	468
6	Pretreatment	Revio	7089	5,007,964	12,584	30	64,251,998	49,524,406	845	8504	5801	818	964
6	Relapse	Revio	7429	5,043,744	11,948	30	62,739,550	46,399,766	804	7449	5001	608	702