DeltaSplice predicts mutations driving lineage-specific splicing in SOX13. SOX13 exon 2 has two alternative 5′ splice sites, as shown in the gene schematics. Multiple-sequence alignments of the alternatively spliced region are shown, together with the usage of the two splice sites in each species on the right. Note that there is insufficient RNA-seq read coverage to quantify SSU in chimpanzees, although the limited number of reads supports the use of 5′ splice site 2. The impact of in silico mutations in each nucleotide position in the region on the two splice sites is shown in the heatmaps at the bottom. The nucleotide base in each position of the human gene sequence is indicated by “x.”