Table 2.

SNV calling in nine-animal pedigree

IndividualsYoungest budded 2018Middle budded 2012Oldest budded 2006Mother
Age (years)0.080.087.207.308.1011.1012.0012.3012.60
# Raw reads193.91151.63290.73304.89302.57281.69301.31304.27191.77
# Mapped reads (million)a146.97114.18199.57209.62206.60191.17200.59217.67143.28
% Mapped readsa80.0879.7672.0271.9871.6570.9669.5274.6379.03
Mother versus buds (2006–2018)
# Downsampled reads (million)141.19141.18141.18141.19141.17141.18141.18141.18-
# Covered basesb442.90444.13440.64436.60446.12444.74442.49444.50-
% Genome coveredb0.520.520.520.510.520.520.520.52-
# Combined variants98113868911110796109-
Individuals versus reference (≪1973 until last sexual reproduction)
# Covered bases (million)b654.78673.32475.15567.36669.09684.24665.69678.00564.77
% Genome coveredb76.6978.8655.6566.4578.3780.1477.9779.4166.15
# Variants (million)4.604.693.383.984.675.214.644.714.75
# Variants in CDS (million)0.180.180.140.160.180.180.180.180.16

[i] aUniquely mapping reads after removal of duplicates.

[ii] b(Coverage ≥10).