MYT1L directly binds to and controls promoters that are associated with early neuronal development genes. (A) Mean MYT1L CUT&RUN signals showed decreased MYT1L binding at promoters in Het PFC. (B) No chromatin accessibility change was observed at MYT1L-bound promoters. (C) Mean H3K4me3 CUT&RUN signals showed increased H3K4me3 at MYT1L-bound (MYT1L⁺) promoters in Het PFC. (D) Mean H3K27ac CUT&RUN signals showed increased H3K27ac at MYT1L-bound promoters in Het PFC. (E) MYT1L coimmunoprecipitated with SIN3B and HDAC2 but not with HDAC1/3/4 and MEF2A in WT mouse cortex. (F) MYT1L loss increased its promoter targets’ expression in PFC. (****) P < 0.0001. (G) Venn diagram showing the overlaps among dDEGs, MYT1L promoter targets, and uDEGs. No biased overlap was observed (P = 0.36). (H) GO analysis on 85 uDEGs whose promoters were bound by MYT1L. (I) Representative genome browser track showed MYT1L-bound Bcl11b promoter had higher H3K4me3 and H3K27ac levels in Het PFC than WT.