MYT1L co-occupies with different sets of TFs at promoter and enhancer regions. (A) monaLisa motif analysis revealed that MYT1L co-occupies with transcriptional activators such as ELK1 at promoter regions, whereas it co-occupies with neurogenic TFs such as MEF2A at enhancer regions. (B) Both MYT1L-bound promoters and enhancers were significantly enriched for MYT1L core binding motif, AAGTT. (C) Overlapping between MYT1L CUT&RUN targets and TFs ChIP-seq peaks showed that more MYT1L promoter targets were also bound by transcriptional activators like SP1 and (D) ELK1 than enhancer targets, whereas more enhancer targets were bound by (E) the neurogenic TF MEF2A and (F) activity-dependent protein JUNB. (G) NEUROD1 and (H) NEUROD2 had stronger presence at MYT1L promoter targets than enhancer targets.