CUT&RUN identifies MYT1L-specific binding targets in E14 mouse cortex and adult mouse PFC. (A) Workflow of CUT&RUN experiments on E14 CTX and adult prefrontal cortex (PFC) created with BioRender (https://www.biorender.com/). (B) Representative Integrative Genomics Viewer (IGV) tracks showing a reproducible MYT1L peak at the Hes1 promoter region in all 3 WT E14 biological replicates but not in IgG and KO samples. (C) Heatmaps of CUT&RUN signals of MYT1L, IgG, and histones at MYT1L-bound regions in PFC. (D) Annotations of PFC ATAC-seq peaks showed the genome-wide distribution of promoters, active enhancers, and poised enhancers in open chromatin regions. (E) Annotations of MYT1L targets in PFC showed MYT1L mainly binds to active enhancers. (F) Representative genome tracks of MYT1L-bound promoter (left), active enhancer (middle), and poised enhancer (right).