Validation of translation of the overlapping lncRNA ORFs between the RNA-seq and Ribo-seq analyses. (A) The relative expression of the selected TP53LC genes and the positive control genes (TINCR and MINION) detected by qRT-PCR assays in HepG2 and HepG2^TP53−/− cells with or without ADR treatment for 24 h. (B) Western blot assays for validating the coding ability of the TP53LC-3xFLAG vectors using the FLAG antibody in HepG2 and SK-HEP-1 cells. The TINCR and MINION vectors were used as positive controls. The pCFH vector was used as a negative control. (C) The protein levels of TP53LC-3xFLAG in HepG2 and SK-HEP-1 cells transfected with the TP53LC-3xFLAG and TP53LC-FS-3xFLAG vectors. (FS) Frameshift mutant. Data are represented as mean ± SEM. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001.