Genome-wide analysis of translated ORFs with the Ribo-seq data of ADR-treated HepG2 cells. (A) Schematic of the experimental procedures of ribosome profiling. (B) RiboCode analysis for 29-nt and 30-nt RPF reads. The P-site positions are color-coded according to the frame. (C) Biotype distribution of all of the translated ORFs identified by the RiboCode method in ADR-treated HepG2 cells (left, ORF ratio; right, ORF numbers). (D) The fraction of putative lncRNA-encoded peptides within different length ranges. (E) Density plots showing the translational efficiency (TE) of translated ORFs between two types of transcripts: the lncRNAs and the annotated protein-coding genes. (F) The conservation of the predicted coding lncRNAs and the annotated protein-coding genes. (G) Distribution of reads for two lncRNA ORFs reported to encode peptides.